Acetosyringone treatment duration affects large T-DNA molecule transfer to rice callus
نویسندگان
چکیده
منابع مشابه
Effect of Acetosyringone and Callus Age on Transformation for Scutellum –derived Callus of Rice
An efficient and reproducible protocol for Agrobacteriummediated transformation of an agronomically useful Oryza sativa variety, Pusa Basmati1 (IET10364) has been standardized. Although several reporters have been claimed the Agrobacteriummediated transformation but here we studied the effect of acetosyringone and age of callus on transformation frequency for scutellumderived callus of Pusa bas...
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Early studies on Agrobacterium tumefaciens showed that development of tumors on plants following infection by A. tumefaciens was optimal at temperatures around 22 degrees C and did not occur at temperatures above 29 degrees C. To assess whether this inability to induce tumors is due to a defect in the T-DNA transfer machinery, mobilization of an incompatibility group Q (IncQ) plasmid by the T-D...
متن کاملMolecular genetics using T-DNA in rice.
Now that sequencing of the rice genome is nearly completed, functional analysis of its large number of genes is the next challenge. Because rice is easy to transform, T-DNA has been used successfully to generate insertional mutant lines. Collectively, several laboratories throughout the world have established at least 200,000 T-DNA insertional lines. Some of those carry the GUS or GFP reporters...
متن کاملCharacterization of two rice DNA methyltransferases and RNAi-mediated restoration of promoter activity in silenced rice callus
Two genomic clones (OsMET1-1, AF 462029 and OsMET1-2), each encoding a cytosine-5 DNA methyltransferase (MTase), were isolated from rice (Oryza sativa L.) BAC libraries. OsMET1-1 has an open reading frame of 4,566 nucleotides with twelve exons and eleven introns while OsMET1-2 has an open reading frame of 4,452 nucleotides with eleven exons and ten introns. Although OsMET1-1 and OsMET1-2 have h...
متن کاملTransfer of T-DNA from Agrobacterium to the plant cell.
Agrobacterium tumefaciens is the causative agent of crown gall, a disease of dicotyledonous plants characterized by a tumorous phenotype. Earlier in this century, scientific interest in A. tumefaciens was based on the possibility that the study of plant tumors might reveal mechanisms that were also operating in animal neoplasia. In the recent past, the tumorous growth was shown to result from t...
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ژورنال
عنوان ژورنال: BMC Biotechnology
سال: 2018
ISSN: 1472-6750
DOI: 10.1186/s12896-018-0459-5